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    Jackson Laboratory utrecht n a mouse
    Utrecht N A Mouse, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/utrecht+n+a+mouse/a+mouse+n+utrecht/pm30917315-234-135-139
    Average 86 stars, based on 1 article reviews
    utrecht n a mouse - by Bioz Stars, 2026-10
    86/100 stars

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    Staining:

    Article Title: Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti-Foxp3 eBiosciences PCH101 Mouse anti-Foxp3 eBiosciences 14-7979; RRID:AB_468499 Mouse anti-FLAG Sigma-Aldrich F7425; RRID:AB_439687 Mouse anti-hemaglutanin (HA) Sigma-Aldrich 12CA5; RRID:AB_514505 Rabbit anti-NLK Santa Cruz H-100; RRID:AB_2112660 Goat anti-actin Santa Cruz I-19; RRID:AB_630836 Anti-HSP90 Cell Signaling 4875; RRID:AB_2233331 Rat anti mouse CD4 (clone RM4-5) Biolegend Cat#100536 Rat anti mouse CD62L (clone MEL-14) Biolegend Cat#104424 Rat anti CD44 (clone IM7) Biolegend Cat#103032 Rat anti mouse CD45Rb (clone 16A) BD PharMingen Cat#553101 Rat anti mouse CD25 (clone PC61) Biolegend Cat#102016 Mouse anti mouse CD45.1 (clone A20) eBiosciences Cat#17-0453-81 Mouse anti mouse CD45.2 (clone 104) eBiosciences Cat#12-0454-81 Rat anti mouse CD8 (clone 53-6.7) Biolegend Cat#100706 Rat anti mouse CD19 (clone 6D5) Biolegend Cat#115538 Rat anti mouse SiglecF (clone E50-2440) BD PharMingen Cat#562681 Rat anti mouse Ly6G (clone 1A8) Biolegend Cat#127628 Rat anti mouse CD3 (clone 17A2) Biolegend Cat#100228 Armenian Hamster anti mouse CD11c (clone N418) Biolegend Cat#117334 Rat anti CD11b (clone M1/70) Biolegend Cat#101241 Rat anti mouse F4/80 (clone BM8) eBiosciences Cat#25-4801-82 Rat anti mouse MHCII (I-A/I-E) (clone M5/ 114.15.2) eBiosciences Cat#47-5321-82 Mouse anti mouse NK-1.1 (clone PK136) Biolegend Cat#108710 Rat anti FoxP3 (clone FJK-16 s) eBiosciences Cat#50-5773-82 Chemicals, Peptides, and Recombinant Proteins Phos-tag acrylamide WAKO Chemicals GmbH 300-93523 DMEM containing GlutMax GIBCO Life Technologies 31966047 RPMI 1640 GIBCO Life Technologies 61870044 IMDM GIBCO Life Technologies 12440-061 FBS GIBCO Life Technologies S1810-500 Penicillin-Streptomycin GIBCO Life Technologies 15140-122 Nonidet p40 US Biologicals N3500 HALT protease inhibitor cocktail Thermo Scientific 78439 Anti-HA conjugated beads Sigma-Aldrich A2095 PVDF Millipore IPFL00010 iScript cDNA synthesis kit BioRad 170-8891 Poly-L-lysine Sigma-Aldrich P4707 Anti-CD3/CD28 Dynabeads ThermoFisher 11452D TGF-b R&D systems 240-B-010 RNasin inhibitor Promega N2111 dNTPs Promega 28-4065-60 (Continued on next page) Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligo dT15 Promega C1101 PEI Polysciences 23966-1 PKB inhibitor VII Calbiochem 124018 5Z-7-oxozeanol Tocris Biosceince 3604/1 BIO Tocris Biosceince 3194/1 gPPAse New England Biolabs P0753s GST-NLK Sigma-Aldrich SRP5285 Okadaic acid Enzo Lifesciences ALX-350-003-C050 SB203580 Enzo Lifesciences BML-EI286-001 SP600125 Enzo Lifesciences BML-EI305-010 Rapamycin Enzo Lifesciences A275-0005 U0126 Enzo Lifesciences BML-EI282-0001 Cycloheximide Sigma-Aldrich C4859 Critical Commercial Assays Foxp3 staining kit eBioscience 00-5521-00 Proximity Ligation Assay kit Sigma-Aldrich DUO92101 Live/dead kit Life Technologies L3224 CellTrace Violet tracker ThermoFisher C34557 Deposited Data Raw and analyzed Mass Spectrometry Data This paper PRIDE: PXD011164 Experimental Models: Cell Lines HEK293 ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57BL/6 Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: BALB/c Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: NLKfl/fl The Jackson Laboratory JAX: 024537 B6;129-Nlktm.1.1Ecan/J Mouse: Foxp3CRE The Jackson Laboratory JAX: 016959 B6.129(Cg)-Foxp3 tm4(YFP/icre)Ayr/J Mouse: Foxp3-eGFP The Jackson Laboratory JAX: 006772 B6.Cg-Foxp3 tm2(EGFP)Tch/J Oligonucleotides See Table S1 N/A Recombinant DNA pMT2-HA_Foxp3 van Loosdregt et al., 2013b N/A pMT2-HA_JNK de Groot et al., 1997 N/A pMT2-HA_ERK de Groot et al., 1997 N/A pMT2-His_Ubi van Loosdregt et al., 2013a N/A pMT2-myc_USP7 van Loosdregt et al., 2013a N/A pMT2-myc_FoxP3 van Loosdregt et al., 2013a N/A pLP/VSVG van Loosdregt et al., 2013a N/A psPAX2 van Loosdregt et al., 2013a N/A pMT2-FLAG_NLK Szypowska et al., 2011 N/A pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000002071 pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000195090 pRFP-C-Rs Nlk shRNA OriGene Technologies TF501483 Stub1 Harvard Medical School HsCD00326610 pUC57 Foxp3 7xA and 7x Genescript Biotech Custom synthesis Software and Algorithms FlowJo 10 FLOWJO, LLC https://www.flowjo.com/ e2 Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 ..

    Proximity Ligation Assay:

    Article Title: Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti-Foxp3 eBiosciences PCH101 Mouse anti-Foxp3 eBiosciences 14-7979; RRID:AB_468499 Mouse anti-FLAG Sigma-Aldrich F7425; RRID:AB_439687 Mouse anti-hemaglutanin (HA) Sigma-Aldrich 12CA5; RRID:AB_514505 Rabbit anti-NLK Santa Cruz H-100; RRID:AB_2112660 Goat anti-actin Santa Cruz I-19; RRID:AB_630836 Anti-HSP90 Cell Signaling 4875; RRID:AB_2233331 Rat anti mouse CD4 (clone RM4-5) Biolegend Cat#100536 Rat anti mouse CD62L (clone MEL-14) Biolegend Cat#104424 Rat anti CD44 (clone IM7) Biolegend Cat#103032 Rat anti mouse CD45Rb (clone 16A) BD PharMingen Cat#553101 Rat anti mouse CD25 (clone PC61) Biolegend Cat#102016 Mouse anti mouse CD45.1 (clone A20) eBiosciences Cat#17-0453-81 Mouse anti mouse CD45.2 (clone 104) eBiosciences Cat#12-0454-81 Rat anti mouse CD8 (clone 53-6.7) Biolegend Cat#100706 Rat anti mouse CD19 (clone 6D5) Biolegend Cat#115538 Rat anti mouse SiglecF (clone E50-2440) BD PharMingen Cat#562681 Rat anti mouse Ly6G (clone 1A8) Biolegend Cat#127628 Rat anti mouse CD3 (clone 17A2) Biolegend Cat#100228 Armenian Hamster anti mouse CD11c (clone N418) Biolegend Cat#117334 Rat anti CD11b (clone M1/70) Biolegend Cat#101241 Rat anti mouse F4/80 (clone BM8) eBiosciences Cat#25-4801-82 Rat anti mouse MHCII (I-A/I-E) (clone M5/ 114.15.2) eBiosciences Cat#47-5321-82 Mouse anti mouse NK-1.1 (clone PK136) Biolegend Cat#108710 Rat anti FoxP3 (clone FJK-16 s) eBiosciences Cat#50-5773-82 Chemicals, Peptides, and Recombinant Proteins Phos-tag acrylamide WAKO Chemicals GmbH 300-93523 DMEM containing GlutMax GIBCO Life Technologies 31966047 RPMI 1640 GIBCO Life Technologies 61870044 IMDM GIBCO Life Technologies 12440-061 FBS GIBCO Life Technologies S1810-500 Penicillin-Streptomycin GIBCO Life Technologies 15140-122 Nonidet p40 US Biologicals N3500 HALT protease inhibitor cocktail Thermo Scientific 78439 Anti-HA conjugated beads Sigma-Aldrich A2095 PVDF Millipore IPFL00010 iScript cDNA synthesis kit BioRad 170-8891 Poly-L-lysine Sigma-Aldrich P4707 Anti-CD3/CD28 Dynabeads ThermoFisher 11452D TGF-b R&D systems 240-B-010 RNasin inhibitor Promega N2111 dNTPs Promega 28-4065-60 (Continued on next page) Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligo dT15 Promega C1101 PEI Polysciences 23966-1 PKB inhibitor VII Calbiochem 124018 5Z-7-oxozeanol Tocris Biosceince 3604/1 BIO Tocris Biosceince 3194/1 gPPAse New England Biolabs P0753s GST-NLK Sigma-Aldrich SRP5285 Okadaic acid Enzo Lifesciences ALX-350-003-C050 SB203580 Enzo Lifesciences BML-EI286-001 SP600125 Enzo Lifesciences BML-EI305-010 Rapamycin Enzo Lifesciences A275-0005 U0126 Enzo Lifesciences BML-EI282-0001 Cycloheximide Sigma-Aldrich C4859 Critical Commercial Assays Foxp3 staining kit eBioscience 00-5521-00 Proximity Ligation Assay kit Sigma-Aldrich DUO92101 Live/dead kit Life Technologies L3224 CellTrace Violet tracker ThermoFisher C34557 Deposited Data Raw and analyzed Mass Spectrometry Data This paper PRIDE: PXD011164 Experimental Models: Cell Lines HEK293 ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57BL/6 Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: BALB/c Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: NLKfl/fl The Jackson Laboratory JAX: 024537 B6;129-Nlktm.1.1Ecan/J Mouse: Foxp3CRE The Jackson Laboratory JAX: 016959 B6.129(Cg)-Foxp3 tm4(YFP/icre)Ayr/J Mouse: Foxp3-eGFP The Jackson Laboratory JAX: 006772 B6.Cg-Foxp3 tm2(EGFP)Tch/J Oligonucleotides See Table S1 N/A Recombinant DNA pMT2-HA_Foxp3 van Loosdregt et al., 2013b N/A pMT2-HA_JNK de Groot et al., 1997 N/A pMT2-HA_ERK de Groot et al., 1997 N/A pMT2-His_Ubi van Loosdregt et al., 2013a N/A pMT2-myc_USP7 van Loosdregt et al., 2013a N/A pMT2-myc_FoxP3 van Loosdregt et al., 2013a N/A pLP/VSVG van Loosdregt et al., 2013a N/A psPAX2 van Loosdregt et al., 2013a N/A pMT2-FLAG_NLK Szypowska et al., 2011 N/A pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000002071 pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000195090 pRFP-C-Rs Nlk shRNA OriGene Technologies TF501483 Stub1 Harvard Medical School HsCD00326610 pUC57 Foxp3 7xA and 7x Genescript Biotech Custom synthesis Software and Algorithms FlowJo 10 FLOWJO, LLC https://www.flowjo.com/ e2 Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 ..

    Mass Spectrometry:

    Article Title: Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti-Foxp3 eBiosciences PCH101 Mouse anti-Foxp3 eBiosciences 14-7979; RRID:AB_468499 Mouse anti-FLAG Sigma-Aldrich F7425; RRID:AB_439687 Mouse anti-hemaglutanin (HA) Sigma-Aldrich 12CA5; RRID:AB_514505 Rabbit anti-NLK Santa Cruz H-100; RRID:AB_2112660 Goat anti-actin Santa Cruz I-19; RRID:AB_630836 Anti-HSP90 Cell Signaling 4875; RRID:AB_2233331 Rat anti mouse CD4 (clone RM4-5) Biolegend Cat#100536 Rat anti mouse CD62L (clone MEL-14) Biolegend Cat#104424 Rat anti CD44 (clone IM7) Biolegend Cat#103032 Rat anti mouse CD45Rb (clone 16A) BD PharMingen Cat#553101 Rat anti mouse CD25 (clone PC61) Biolegend Cat#102016 Mouse anti mouse CD45.1 (clone A20) eBiosciences Cat#17-0453-81 Mouse anti mouse CD45.2 (clone 104) eBiosciences Cat#12-0454-81 Rat anti mouse CD8 (clone 53-6.7) Biolegend Cat#100706 Rat anti mouse CD19 (clone 6D5) Biolegend Cat#115538 Rat anti mouse SiglecF (clone E50-2440) BD PharMingen Cat#562681 Rat anti mouse Ly6G (clone 1A8) Biolegend Cat#127628 Rat anti mouse CD3 (clone 17A2) Biolegend Cat#100228 Armenian Hamster anti mouse CD11c (clone N418) Biolegend Cat#117334 Rat anti CD11b (clone M1/70) Biolegend Cat#101241 Rat anti mouse F4/80 (clone BM8) eBiosciences Cat#25-4801-82 Rat anti mouse MHCII (I-A/I-E) (clone M5/ 114.15.2) eBiosciences Cat#47-5321-82 Mouse anti mouse NK-1.1 (clone PK136) Biolegend Cat#108710 Rat anti FoxP3 (clone FJK-16 s) eBiosciences Cat#50-5773-82 Chemicals, Peptides, and Recombinant Proteins Phos-tag acrylamide WAKO Chemicals GmbH 300-93523 DMEM containing GlutMax GIBCO Life Technologies 31966047 RPMI 1640 GIBCO Life Technologies 61870044 IMDM GIBCO Life Technologies 12440-061 FBS GIBCO Life Technologies S1810-500 Penicillin-Streptomycin GIBCO Life Technologies 15140-122 Nonidet p40 US Biologicals N3500 HALT protease inhibitor cocktail Thermo Scientific 78439 Anti-HA conjugated beads Sigma-Aldrich A2095 PVDF Millipore IPFL00010 iScript cDNA synthesis kit BioRad 170-8891 Poly-L-lysine Sigma-Aldrich P4707 Anti-CD3/CD28 Dynabeads ThermoFisher 11452D TGF-b R&D systems 240-B-010 RNasin inhibitor Promega N2111 dNTPs Promega 28-4065-60 (Continued on next page) Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligo dT15 Promega C1101 PEI Polysciences 23966-1 PKB inhibitor VII Calbiochem 124018 5Z-7-oxozeanol Tocris Biosceince 3604/1 BIO Tocris Biosceince 3194/1 gPPAse New England Biolabs P0753s GST-NLK Sigma-Aldrich SRP5285 Okadaic acid Enzo Lifesciences ALX-350-003-C050 SB203580 Enzo Lifesciences BML-EI286-001 SP600125 Enzo Lifesciences BML-EI305-010 Rapamycin Enzo Lifesciences A275-0005 U0126 Enzo Lifesciences BML-EI282-0001 Cycloheximide Sigma-Aldrich C4859 Critical Commercial Assays Foxp3 staining kit eBioscience 00-5521-00 Proximity Ligation Assay kit Sigma-Aldrich DUO92101 Live/dead kit Life Technologies L3224 CellTrace Violet tracker ThermoFisher C34557 Deposited Data Raw and analyzed Mass Spectrometry Data This paper PRIDE: PXD011164 Experimental Models: Cell Lines HEK293 ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57BL/6 Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: BALB/c Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: NLKfl/fl The Jackson Laboratory JAX: 024537 B6;129-Nlktm.1.1Ecan/J Mouse: Foxp3CRE The Jackson Laboratory JAX: 016959 B6.129(Cg)-Foxp3 tm4(YFP/icre)Ayr/J Mouse: Foxp3-eGFP The Jackson Laboratory JAX: 006772 B6.Cg-Foxp3 tm2(EGFP)Tch/J Oligonucleotides See Table S1 N/A Recombinant DNA pMT2-HA_Foxp3 van Loosdregt et al., 2013b N/A pMT2-HA_JNK de Groot et al., 1997 N/A pMT2-HA_ERK de Groot et al., 1997 N/A pMT2-His_Ubi van Loosdregt et al., 2013a N/A pMT2-myc_USP7 van Loosdregt et al., 2013a N/A pMT2-myc_FoxP3 van Loosdregt et al., 2013a N/A pLP/VSVG van Loosdregt et al., 2013a N/A psPAX2 van Loosdregt et al., 2013a N/A pMT2-FLAG_NLK Szypowska et al., 2011 N/A pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000002071 pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000195090 pRFP-C-Rs Nlk shRNA OriGene Technologies TF501483 Stub1 Harvard Medical School HsCD00326610 pUC57 Foxp3 7xA and 7x Genescript Biotech Custom synthesis Software and Algorithms FlowJo 10 FLOWJO, LLC https://www.flowjo.com/ e2 Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 ..

    Recombinant:

    Article Title: Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti-Foxp3 eBiosciences PCH101 Mouse anti-Foxp3 eBiosciences 14-7979; RRID:AB_468499 Mouse anti-FLAG Sigma-Aldrich F7425; RRID:AB_439687 Mouse anti-hemaglutanin (HA) Sigma-Aldrich 12CA5; RRID:AB_514505 Rabbit anti-NLK Santa Cruz H-100; RRID:AB_2112660 Goat anti-actin Santa Cruz I-19; RRID:AB_630836 Anti-HSP90 Cell Signaling 4875; RRID:AB_2233331 Rat anti mouse CD4 (clone RM4-5) Biolegend Cat#100536 Rat anti mouse CD62L (clone MEL-14) Biolegend Cat#104424 Rat anti CD44 (clone IM7) Biolegend Cat#103032 Rat anti mouse CD45Rb (clone 16A) BD PharMingen Cat#553101 Rat anti mouse CD25 (clone PC61) Biolegend Cat#102016 Mouse anti mouse CD45.1 (clone A20) eBiosciences Cat#17-0453-81 Mouse anti mouse CD45.2 (clone 104) eBiosciences Cat#12-0454-81 Rat anti mouse CD8 (clone 53-6.7) Biolegend Cat#100706 Rat anti mouse CD19 (clone 6D5) Biolegend Cat#115538 Rat anti mouse SiglecF (clone E50-2440) BD PharMingen Cat#562681 Rat anti mouse Ly6G (clone 1A8) Biolegend Cat#127628 Rat anti mouse CD3 (clone 17A2) Biolegend Cat#100228 Armenian Hamster anti mouse CD11c (clone N418) Biolegend Cat#117334 Rat anti CD11b (clone M1/70) Biolegend Cat#101241 Rat anti mouse F4/80 (clone BM8) eBiosciences Cat#25-4801-82 Rat anti mouse MHCII (I-A/I-E) (clone M5/ 114.15.2) eBiosciences Cat#47-5321-82 Mouse anti mouse NK-1.1 (clone PK136) Biolegend Cat#108710 Rat anti FoxP3 (clone FJK-16 s) eBiosciences Cat#50-5773-82 Chemicals, Peptides, and Recombinant Proteins Phos-tag acrylamide WAKO Chemicals GmbH 300-93523 DMEM containing GlutMax GIBCO Life Technologies 31966047 RPMI 1640 GIBCO Life Technologies 61870044 IMDM GIBCO Life Technologies 12440-061 FBS GIBCO Life Technologies S1810-500 Penicillin-Streptomycin GIBCO Life Technologies 15140-122 Nonidet p40 US Biologicals N3500 HALT protease inhibitor cocktail Thermo Scientific 78439 Anti-HA conjugated beads Sigma-Aldrich A2095 PVDF Millipore IPFL00010 iScript cDNA synthesis kit BioRad 170-8891 Poly-L-lysine Sigma-Aldrich P4707 Anti-CD3/CD28 Dynabeads ThermoFisher 11452D TGF-b R&D systems 240-B-010 RNasin inhibitor Promega N2111 dNTPs Promega 28-4065-60 (Continued on next page) Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligo dT15 Promega C1101 PEI Polysciences 23966-1 PKB inhibitor VII Calbiochem 124018 5Z-7-oxozeanol Tocris Biosceince 3604/1 BIO Tocris Biosceince 3194/1 gPPAse New England Biolabs P0753s GST-NLK Sigma-Aldrich SRP5285 Okadaic acid Enzo Lifesciences ALX-350-003-C050 SB203580 Enzo Lifesciences BML-EI286-001 SP600125 Enzo Lifesciences BML-EI305-010 Rapamycin Enzo Lifesciences A275-0005 U0126 Enzo Lifesciences BML-EI282-0001 Cycloheximide Sigma-Aldrich C4859 Critical Commercial Assays Foxp3 staining kit eBioscience 00-5521-00 Proximity Ligation Assay kit Sigma-Aldrich DUO92101 Live/dead kit Life Technologies L3224 CellTrace Violet tracker ThermoFisher C34557 Deposited Data Raw and analyzed Mass Spectrometry Data This paper PRIDE: PXD011164 Experimental Models: Cell Lines HEK293 ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57BL/6 Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: BALB/c Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: NLKfl/fl The Jackson Laboratory JAX: 024537 B6;129-Nlktm.1.1Ecan/J Mouse: Foxp3CRE The Jackson Laboratory JAX: 016959 B6.129(Cg)-Foxp3 tm4(YFP/icre)Ayr/J Mouse: Foxp3-eGFP The Jackson Laboratory JAX: 006772 B6.Cg-Foxp3 tm2(EGFP)Tch/J Oligonucleotides See Table S1 N/A Recombinant DNA pMT2-HA_Foxp3 van Loosdregt et al., 2013b N/A pMT2-HA_JNK de Groot et al., 1997 N/A pMT2-HA_ERK de Groot et al., 1997 N/A pMT2-His_Ubi van Loosdregt et al., 2013a N/A pMT2-myc_USP7 van Loosdregt et al., 2013a N/A pMT2-myc_FoxP3 van Loosdregt et al., 2013a N/A pLP/VSVG van Loosdregt et al., 2013a N/A psPAX2 van Loosdregt et al., 2013a N/A pMT2-FLAG_NLK Szypowska et al., 2011 N/A pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000002071 pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000195090 pRFP-C-Rs Nlk shRNA OriGene Technologies TF501483 Stub1 Harvard Medical School HsCD00326610 pUC57 Foxp3 7xA and 7x Genescript Biotech Custom synthesis Software and Algorithms FlowJo 10 FLOWJO, LLC https://www.flowjo.com/ e2 Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 ..

    shRNA:

    Article Title: Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti-Foxp3 eBiosciences PCH101 Mouse anti-Foxp3 eBiosciences 14-7979; RRID:AB_468499 Mouse anti-FLAG Sigma-Aldrich F7425; RRID:AB_439687 Mouse anti-hemaglutanin (HA) Sigma-Aldrich 12CA5; RRID:AB_514505 Rabbit anti-NLK Santa Cruz H-100; RRID:AB_2112660 Goat anti-actin Santa Cruz I-19; RRID:AB_630836 Anti-HSP90 Cell Signaling 4875; RRID:AB_2233331 Rat anti mouse CD4 (clone RM4-5) Biolegend Cat#100536 Rat anti mouse CD62L (clone MEL-14) Biolegend Cat#104424 Rat anti CD44 (clone IM7) Biolegend Cat#103032 Rat anti mouse CD45Rb (clone 16A) BD PharMingen Cat#553101 Rat anti mouse CD25 (clone PC61) Biolegend Cat#102016 Mouse anti mouse CD45.1 (clone A20) eBiosciences Cat#17-0453-81 Mouse anti mouse CD45.2 (clone 104) eBiosciences Cat#12-0454-81 Rat anti mouse CD8 (clone 53-6.7) Biolegend Cat#100706 Rat anti mouse CD19 (clone 6D5) Biolegend Cat#115538 Rat anti mouse SiglecF (clone E50-2440) BD PharMingen Cat#562681 Rat anti mouse Ly6G (clone 1A8) Biolegend Cat#127628 Rat anti mouse CD3 (clone 17A2) Biolegend Cat#100228 Armenian Hamster anti mouse CD11c (clone N418) Biolegend Cat#117334 Rat anti CD11b (clone M1/70) Biolegend Cat#101241 Rat anti mouse F4/80 (clone BM8) eBiosciences Cat#25-4801-82 Rat anti mouse MHCII (I-A/I-E) (clone M5/ 114.15.2) eBiosciences Cat#47-5321-82 Mouse anti mouse NK-1.1 (clone PK136) Biolegend Cat#108710 Rat anti FoxP3 (clone FJK-16 s) eBiosciences Cat#50-5773-82 Chemicals, Peptides, and Recombinant Proteins Phos-tag acrylamide WAKO Chemicals GmbH 300-93523 DMEM containing GlutMax GIBCO Life Technologies 31966047 RPMI 1640 GIBCO Life Technologies 61870044 IMDM GIBCO Life Technologies 12440-061 FBS GIBCO Life Technologies S1810-500 Penicillin-Streptomycin GIBCO Life Technologies 15140-122 Nonidet p40 US Biologicals N3500 HALT protease inhibitor cocktail Thermo Scientific 78439 Anti-HA conjugated beads Sigma-Aldrich A2095 PVDF Millipore IPFL00010 iScript cDNA synthesis kit BioRad 170-8891 Poly-L-lysine Sigma-Aldrich P4707 Anti-CD3/CD28 Dynabeads ThermoFisher 11452D TGF-b R&D systems 240-B-010 RNasin inhibitor Promega N2111 dNTPs Promega 28-4065-60 (Continued on next page) Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligo dT15 Promega C1101 PEI Polysciences 23966-1 PKB inhibitor VII Calbiochem 124018 5Z-7-oxozeanol Tocris Biosceince 3604/1 BIO Tocris Biosceince 3194/1 gPPAse New England Biolabs P0753s GST-NLK Sigma-Aldrich SRP5285 Okadaic acid Enzo Lifesciences ALX-350-003-C050 SB203580 Enzo Lifesciences BML-EI286-001 SP600125 Enzo Lifesciences BML-EI305-010 Rapamycin Enzo Lifesciences A275-0005 U0126 Enzo Lifesciences BML-EI282-0001 Cycloheximide Sigma-Aldrich C4859 Critical Commercial Assays Foxp3 staining kit eBioscience 00-5521-00 Proximity Ligation Assay kit Sigma-Aldrich DUO92101 Live/dead kit Life Technologies L3224 CellTrace Violet tracker ThermoFisher C34557 Deposited Data Raw and analyzed Mass Spectrometry Data This paper PRIDE: PXD011164 Experimental Models: Cell Lines HEK293 ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57BL/6 Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: BALB/c Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: NLKfl/fl The Jackson Laboratory JAX: 024537 B6;129-Nlktm.1.1Ecan/J Mouse: Foxp3CRE The Jackson Laboratory JAX: 016959 B6.129(Cg)-Foxp3 tm4(YFP/icre)Ayr/J Mouse: Foxp3-eGFP The Jackson Laboratory JAX: 006772 B6.Cg-Foxp3 tm2(EGFP)Tch/J Oligonucleotides See Table S1 N/A Recombinant DNA pMT2-HA_Foxp3 van Loosdregt et al., 2013b N/A pMT2-HA_JNK de Groot et al., 1997 N/A pMT2-HA_ERK de Groot et al., 1997 N/A pMT2-His_Ubi van Loosdregt et al., 2013a N/A pMT2-myc_USP7 van Loosdregt et al., 2013a N/A pMT2-myc_FoxP3 van Loosdregt et al., 2013a N/A pLP/VSVG van Loosdregt et al., 2013a N/A psPAX2 van Loosdregt et al., 2013a N/A pMT2-FLAG_NLK Szypowska et al., 2011 N/A pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000002071 pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000195090 pRFP-C-Rs Nlk shRNA OriGene Technologies TF501483 Stub1 Harvard Medical School HsCD00326610 pUC57 Foxp3 7xA and 7x Genescript Biotech Custom synthesis Software and Algorithms FlowJo 10 FLOWJO, LLC https://www.flowjo.com/ e2 Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 ..

    Software:

    Article Title: Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti-Foxp3 eBiosciences PCH101 Mouse anti-Foxp3 eBiosciences 14-7979; RRID:AB_468499 Mouse anti-FLAG Sigma-Aldrich F7425; RRID:AB_439687 Mouse anti-hemaglutanin (HA) Sigma-Aldrich 12CA5; RRID:AB_514505 Rabbit anti-NLK Santa Cruz H-100; RRID:AB_2112660 Goat anti-actin Santa Cruz I-19; RRID:AB_630836 Anti-HSP90 Cell Signaling 4875; RRID:AB_2233331 Rat anti mouse CD4 (clone RM4-5) Biolegend Cat#100536 Rat anti mouse CD62L (clone MEL-14) Biolegend Cat#104424 Rat anti CD44 (clone IM7) Biolegend Cat#103032 Rat anti mouse CD45Rb (clone 16A) BD PharMingen Cat#553101 Rat anti mouse CD25 (clone PC61) Biolegend Cat#102016 Mouse anti mouse CD45.1 (clone A20) eBiosciences Cat#17-0453-81 Mouse anti mouse CD45.2 (clone 104) eBiosciences Cat#12-0454-81 Rat anti mouse CD8 (clone 53-6.7) Biolegend Cat#100706 Rat anti mouse CD19 (clone 6D5) Biolegend Cat#115538 Rat anti mouse SiglecF (clone E50-2440) BD PharMingen Cat#562681 Rat anti mouse Ly6G (clone 1A8) Biolegend Cat#127628 Rat anti mouse CD3 (clone 17A2) Biolegend Cat#100228 Armenian Hamster anti mouse CD11c (clone N418) Biolegend Cat#117334 Rat anti CD11b (clone M1/70) Biolegend Cat#101241 Rat anti mouse F4/80 (clone BM8) eBiosciences Cat#25-4801-82 Rat anti mouse MHCII (I-A/I-E) (clone M5/ 114.15.2) eBiosciences Cat#47-5321-82 Mouse anti mouse NK-1.1 (clone PK136) Biolegend Cat#108710 Rat anti FoxP3 (clone FJK-16 s) eBiosciences Cat#50-5773-82 Chemicals, Peptides, and Recombinant Proteins Phos-tag acrylamide WAKO Chemicals GmbH 300-93523 DMEM containing GlutMax GIBCO Life Technologies 31966047 RPMI 1640 GIBCO Life Technologies 61870044 IMDM GIBCO Life Technologies 12440-061 FBS GIBCO Life Technologies S1810-500 Penicillin-Streptomycin GIBCO Life Technologies 15140-122 Nonidet p40 US Biologicals N3500 HALT protease inhibitor cocktail Thermo Scientific 78439 Anti-HA conjugated beads Sigma-Aldrich A2095 PVDF Millipore IPFL00010 iScript cDNA synthesis kit BioRad 170-8891 Poly-L-lysine Sigma-Aldrich P4707 Anti-CD3/CD28 Dynabeads ThermoFisher 11452D TGF-b R&D systems 240-B-010 RNasin inhibitor Promega N2111 dNTPs Promega 28-4065-60 (Continued on next page) Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Oligo dT15 Promega C1101 PEI Polysciences 23966-1 PKB inhibitor VII Calbiochem 124018 5Z-7-oxozeanol Tocris Biosceince 3604/1 BIO Tocris Biosceince 3194/1 gPPAse New England Biolabs P0753s GST-NLK Sigma-Aldrich SRP5285 Okadaic acid Enzo Lifesciences ALX-350-003-C050 SB203580 Enzo Lifesciences BML-EI286-001 SP600125 Enzo Lifesciences BML-EI305-010 Rapamycin Enzo Lifesciences A275-0005 U0126 Enzo Lifesciences BML-EI282-0001 Cycloheximide Sigma-Aldrich C4859 Critical Commercial Assays Foxp3 staining kit eBioscience 00-5521-00 Proximity Ligation Assay kit Sigma-Aldrich DUO92101 Live/dead kit Life Technologies L3224 CellTrace Violet tracker ThermoFisher C34557 Deposited Data Raw and analyzed Mass Spectrometry Data This paper PRIDE: PXD011164 Experimental Models: Cell Lines HEK293 ATCC CRL-11268 Experimental Models: Organisms/Strains Mouse: C57BL/6 Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: BALB/c Internal breeding colonies of the University of Edinburgh or of the University of Utrecht N/A Mouse: NLKfl/fl The Jackson Laboratory JAX: 024537 B6;129-Nlktm.1.1Ecan/J Mouse: Foxp3CRE The Jackson Laboratory JAX: 016959 B6.129(Cg)-Foxp3 tm4(YFP/icre)Ayr/J Mouse: Foxp3-eGFP The Jackson Laboratory JAX: 006772 B6.Cg-Foxp3 tm2(EGFP)Tch/J Oligonucleotides See Table S1 N/A Recombinant DNA pMT2-HA_Foxp3 van Loosdregt et al., 2013b N/A pMT2-HA_JNK de Groot et al., 1997 N/A pMT2-HA_ERK de Groot et al., 1997 N/A pMT2-His_Ubi van Loosdregt et al., 2013a N/A pMT2-myc_USP7 van Loosdregt et al., 2013a N/A pMT2-myc_FoxP3 van Loosdregt et al., 2013a N/A pLP/VSVG van Loosdregt et al., 2013a N/A psPAX2 van Loosdregt et al., 2013a N/A pMT2-FLAG_NLK Szypowska et al., 2011 N/A pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000002071 pLKO.1 puro NLK human shRNA Sigma-Aldrich TRCN0000195090 pRFP-C-Rs Nlk shRNA OriGene Technologies TF501483 Stub1 Harvard Medical School HsCD00326610 pUC57 Foxp3 7xA and 7x Genescript Biotech Custom synthesis Software and Algorithms FlowJo 10 FLOWJO, LLC https://www.flowjo.com/ e2 Cell Reports 26, 3600–3612.e1–e6, March 26, 2019 ..



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